View Data: Functional Assessment of p53 Mutant Proteins in Various Experimental Assays
| Function_ID | ProtDescription | AAchange | Codon_Number | WT_AA | Mutant_AA | SwissProtLink | Structural_motif | Codon72AA | Conserved_WT_Function | Loss_of_Function | Dominant_Negative_Activity | Gain_of_Function | Temperature_Sensitivity | Temp_ref | Cell_assay | Cell_lines | Assay_design | Method | FRef_ID | Authors | Title | Year | Journal | Volume | Start_page | PubMed | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Function_ID | ProtDescription | AAchange | Codon_Number | WT_AA | Mutant_AA | SwissProtLink | Structural_motif | Codon72AA | Conserved_WT_Function | Loss_of_Function | Dominant_Negative_Activity | Gain_of_Function | Temperature_Sensitivity | Temp_ref | Cell_assay | Cell_lines | Assay_design | Method | FRef_ID | Authors | Title | Year | Journal | Volume | Start_page | PubMed | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | p.V143A | V143A | 143 | Val | Ala | 005887 | NDBL/beta-sheets | R | DNAb (p53CON) | TA (p53CON) partial; TA (RGC) | NA | NA | NA | 37°C | Human | K-562 | WT control | Luciferase assay, EMSA | 1 | Zhang W;Funk WD;Wright WE;Shay JW;Deisseroth AB; | Novel DNA binding of p53 mutants and their role in transcriptional activation | 1993 | Oncogene | 8 | 2555 | 8361764 | |
| 2 | p.R175H | R175H | 175 | Arg | His | 005932 | L2/L3 | NA | DNAb (p53CON) | TA (p53CON, RGC) | NA | NA | NA | 37°C | Human | K-562 | WT control | Luciferase assay, EMSA | 1 | Zhang W;Funk WD;Wright WE;Shay JW;Deisseroth AB; | Novel DNA binding of p53 mutants and their role in transcriptional activation | 1993 | Oncogene | 8 | 2555 | 8361764 | |
| 6 | p.R248W | R248W | 248 | Arg | Trp | 005984 | L2/L3 | NA | NA | DNAb (p53CON); TA (p53CON, RGC) | NA | NA | NA | 37°C | Human | K-562 | WT control | Luciferase assay, EMSA | 1 | Zhang W;Funk WD;Wright WE;Shay JW;Deisseroth AB; | Novel DNA binding of p53 mutants and their role in transcriptional activation | 1993 | Oncogene | 8 | 2555 | 8361764 | |
| 7 | p.R273H | R273H | 273 | Arg | His | 005995 | L1/S/H2 | NA | DNAb (p53CON) | TA (p53CON) partial; TA (RGC) | NA | NA | NA | 37°C | Human | K-562 | WT control | Luciferase assay, EMSA | 1 | Zhang W;Funk WD;Wright WE;Shay JW;Deisseroth AB; | Novel DNA binding of p53 mutants and their role in transcriptional activation | 1993 | Oncogene | 8 | 2555 | 8361764 | |
| 8 | p.D281G | D281G | 281 | Asp | Gly | 006012 | L1/S/H2 | NA | NA | DNAb (p53CON); TA (p53CON, RGC) | NA | NA | NA | 37°C | Human | K-562 | WT control | Luciferase assay, EMSA | 1 | Zhang W;Funk WD;Wright WE;Shay JW;Deisseroth AB; | Novel DNA binding of p53 mutants and their role in transcriptional activation | 1993 | Oncogene | 8 | 2555 | 8361764 | |
| 9 | p.R175H | R175H | 175 | Arg | His | 005932 | L2/L3 | NA | NA | NA | NA | Upregulation (dUTPase, C-MYC) | NA | 37°C | Human | H1299, SK-OV-3 | WT control | RT-PCR | 2 | Pugacheva EN;Ivanov AV;Kravchenko JE;Kopnin BP;Levine AJ;Chumakov PM; | Novel gain of function activity of p53 mutants: activation of the dUTPase gene expression leading to resistance to 5-fluorouracil | 2002 | Oncogene | 21 | 4595 | 12096336 | |
| 10 | p.R248W | R248W | 248 | Arg | Trp | 005984 | L2/L3 | NA | NA | NA | NA | Upregulation (dUTPase, C-MYC) | NA | 37°C | Human | H1299, SK-OV-3 | WT control | RT-PCR | 2 | Pugacheva EN;Ivanov AV;Kravchenko JE;Kopnin BP;Levine AJ;Chumakov PM; | Novel gain of function activity of p53 mutants: activation of the dUTPase gene expression leading to resistance to 5-fluorouracil | 2002 | Oncogene | 21 | 4595 | 12096336 | |
| 11 | p.R273H | R273H | 273 | Arg | His | 005995 | L1/S/H2 | NA | NA | NA | NA | Upregulation (C-MYC); no [upregulation (dUTPase)] | NA | 37°C | Human | H1299, SK-OV-3 | WT control | RT-PCR | 2 | Pugacheva EN;Ivanov AV;Kravchenko JE;Kopnin BP;Levine AJ;Chumakov PM; | Novel gain of function activity of p53 mutants: activation of the dUTPase gene expression leading to resistance to 5-fluorouracil | 2002 | Oncogene | 21 | 4595 | 12096336 | |
| 12 | p.D281G | D281G | 281 | Asp | Gly | 006012 | L1/S/H2 | NA | APO | Upregulation (fas, bax); upregulation (WAF1); GA | NA | APO independent of Bcl-2/BAX | NA | 37°C | Human | PC-3 | WT control | FACS analysis, western blot | 3 | He M;Rennie PS;Dragowska V;Nelson CC;Jia W; | A mutant P53 can activate apoptosis through a mechanism distinct from those induced by wild type P53 | 2002 | FEBS Lett | 517 | 151 | 12062426 | |
| 13 | p.D281G | D281G | 281 | Asp | Gly | 006012 | L1/S/H2 | NA | NA | NA | NA | TA (HsMAD1) | NA | 37°C | Human | HeLa, HCT-116, HCT-116 (p53-null) | WT control | Luciferase assay | 4 | Iwanaga Y;Jeang KT; | Expression of mitotic spindle checkpoint protein hsMAD1 correlates with cellular proliferation and is activated by a gain-of-function p53 mutant | 2002 | Cancer Res | 62 | 2618 | 11980658 |