# The following primers have been used in Fernandez-Cuesta et al., (2016) Identification of Circulating Tumor DNA for the Early Detection of Small-cell Lung Cancer.

Gene_Primer regions	Primer sequences
TP53_Exon_2A_F	CACTCACAGTTTCCATAGGTCTG
TP53_Exon_2A_R	GGTTGGAAGTGTCTCATGCTG
TP53_Exon_2B_F	GGAATTTTCGCTTCCCACAG
TP53_Exon_2B_R	AGTCAGATCCTAGCGTCGAGC
TP53_Exon_3_F	TGTCCTTACCAGAACGTTGTT
TP53_Exon_3_R	GGATCCATTGGAAGGGCAG
TP53_Exon_4A_F	CCTCTGGCATTCTGGGAGC
TP53_Exon_4A_R	CTGCTCTTTTCACCCATCTAC
TP53_Exon_4B_F	CGGTGTAGGAGCTGCTG
TP53_Exon_4B_R	TGCCGTCCCAAGCAATG
TP53_Exon_4C_F	GGAAACCGTAGCTGCCCTG
TP53_Exon_4C_R	ACCCAGGTCCAGATGAAG
TP53_Exon_4D_F	TGAAGTCTCATGGAAGCCAGC
TP53_Exon_4D_R	TCCTGGCCCCTGTCATC
TP53_Exon_5A_F	TGGAATCAACCCACAGC
TP53_Exon_5A_R	CTGCCGTCTTCCAGTTGC
TP53_Exon_5B_F	AGCTGCTCACCATCGCTATC
TP53_Exon_5B_R	CCAAGACCTGCCCTGTG
TP53_Exon_5C_F	GCAATCAGTGAGGAATCAGAG
TP53_Exon_5C_R	GCCATCTACAAGCAGTCACAG
TP53_Exon_6_F	CCCAGTTGCAAACCAGAC
TP53_Exon_6_R	GATTCCTCACTGATTGCTCTTAG
TP53_Exon_7A_F	AGTGGCTCCTGACCTGGAGTC
TP53_Exon_7A_R	ATCTTGGGCCTGTGTTATCTC
TP53_Exon_7B_F	GTGGCAAGTGGCTCCTG
TP53_Exon_7B_R	GGCTCTGACTGTACCACCATC
TP53_Exon_8A_F	AGCTCGTGGTGAGGCTC
TP53_Exon_8A_R	TTCCTTACTGCCTCTTGCTTC
TP53_Exon_8B_F	CTTCTTGTCCTGCTTGCTTAC
TP53_Exon_8B_R	GGACGGAACAGCTTTGAG
TP53_Exon_9A_F	TATTCTCCATCCAGTGGTTTC
TP53_Exon_9A_R	GCAGTTATGCCTCAGATTCAC
TP53_Exon_9B_F	AAACTTTCCACTTGATAAGAGGTC
TP53_Exon_9B_R	TCCTTGCCTCTTTCCTAGCAC
TP53_Exon_10A_F	ACCTGGAGTGAGCCCTG
TP53_Exon_10A_R	CCCTCCTCTGTTGCTGC
TP53_Exon_10B_F	GAATGGAATCCTATGGCTTTC
TP53_Exon_10B_R	TGGAACTCAAGGATGCCCAG
TP53_Exon_11A_F	GTGGGAGGCTGTCAGTG
TP53_Exon_11A_R	CTCCCTGCTTCTGTCTCCTAC
TP53_Exon_11B_F	CAAATGGAAGTCCTGGGTGC
TP53_Exon_11B_R	ACAGAAGGGCCTGACTCAGAC